Suppressive Effects of Welsh Onion Extracts on Mucus Hyper-Production in Human Airway Cells

We investigated the suppressive effects of Welsh onion (Allium fistulosum L.) on the hyper-production of mucins in NCI-H292 human lung cancer cells. Periodic acid-Schiff staining showed that the 50% (v/v) ethanol extract of Welsh onion suppressed mucus glycoprotein production in cells, whereas the water and ethanol extracts did not. A real-time RT-PCR analysis demonstrated that the 50% ethanol extract attenuated the gene expression of MUC2 and MUC5AC, which are major airway mucus components, in NCI-H292 cells stimulated with lipopolysaccharide (LPS). Furthermore, dot blot hybridization revealed that the extract at 100 μg/ml attenuated the hyper-production of the MUC5AC protein in LPS-stimulated NCI-H292 cells. *Corresponding author: Akiko Takashima, Sano Inc., 3-4-2 Oroshi-machi, Akita, 010-0061, Japan, Tel: +81-18-862-6644; Fax: +81-18-862-6646; E-mail: [email protected] Received Date: Sept 6, 2016 Accepted Date: Sept 12, 2016 Published Date: Sept 17, 2016 Citation: Takashima, A., et al. Suppressive Effects of Welsh Onion Extracts on Mucus Hyper-Production in Human Airway Cells. (2016) Lett Health Biol Sci 1(2): 3436 Lett Health Biol Sci | volume 1: issue 2 www.ommegaonline.org